The regulatable MAL32 promoter in S. cerevisiae: characteristics and tools
نویسندگان
چکیده
Matthias Meurer, Veronika Chevyreva, Bram Cerulus and Michael Knop Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany. KU Leuven Department Microbiële en Moleculaire Systemen, CMPG Laboratory of Genetics and Genomics, Gaston Geenslaan 1, 3001 Leuven, Belgium VIB Laboratory of Systems Biology, Gaston Geenslaan 1, 3001 Leuven, Belgium Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. Corresponding author: [email protected]
منابع مشابه
The regulatable MAL32 promoter in S. cerevisiae: characteristics and tools to facilitate its use
Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independentl...
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تاریخ انتشار 2016